PREY

CHMP2B

ALS17, CHMP2.5, DMT1, VPS2-2, VPS2B, CGI-84
charged multivesicular body protein 2B
Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Ab initio protein modelling reveals novel human MIT domains.

Rigden DJ, Liu H, Hayes SD, Urbe S, Clague MJ

Database searches can fail to detect all truly homologous sequences, particularly when dealing with short, highly sequence diverse protein families. Here, using microtubule interacting and transport (MIT) domains as an example, we have applied an approach of profile-profile matching followed by ab initio structure modelling to the detection of true homologues in the borderline significant zone of database searches. Novel ... [more]

FEBS Lett. Mar. 04, 2009; 583(5);872-8 [Pubmed: 19302785]

Throughput

  • Low Throughput

Curated By

  • BioGRID