Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

A protein interaction network for Ecm29 links the 26 S proteasome to molecular motors and endosomal components.

Gorbea C, Pratt G, Ustrell V, Bell R, Sahasrabudhe S, Hughes RE, Rechsteiner M

Ecm29 is a 200-kDa HEAT repeat protein that binds the 26 S proteasome. Genome-wide two-hybrid screens and mass spectrometry have identified molecular motors, endosomal components, and ubiquitin-proteasome factors as Ecm29-interacting proteins. The C-terminal half of human Ecm29 binds myosins and kinesins; its N-terminal region binds the endocytic proteins, Vps11, Rab11-FIP4, and rabaptin. Whereas full-length FLAG-Ecm29, its C-terminal half, and a ... [more]

J. Biol. Chem. Oct. 08, 2010; 285(41);31616-33 [Pubmed: 20682791]

Throughput

High Throughput

Additional Notes

figure 1, table S2,S3.

Curated By

BioGRID

Interaction Summary

DST

Gene Ontology Biological Process

Gene Ontology Molecular Function

integrin binding [IPI]microtubule plus-end binding [IDA]protein C-terminus binding [IPI]protein binding [IPI]protein homodimerization activity [IDA]

Gene Ontology Cellular Component

KIAA0368