Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

A protein interaction network for Ecm29 links the 26 S proteasome to molecular motors and endosomal components.

Gorbea C, Pratt G, Ustrell V, Bell R, Sahasrabudhe S, Hughes RE, Rechsteiner M

Ecm29 is a 200-kDa HEAT repeat protein that binds the 26 S proteasome. Genome-wide two-hybrid screens and mass spectrometry have identified molecular motors, endosomal components, and ubiquitin-proteasome factors as Ecm29-interacting proteins. The C-terminal half of human Ecm29 binds myosins and kinesins; its N-terminal region binds the endocytic proteins, Vps11, Rab11-FIP4, and rabaptin. Whereas full-length FLAG-Ecm29, its C-terminal half, and a ... [more]

J. Biol. Chem. Oct. 08, 2010; 285(41);31616-33 [Pubmed: 20682791]

Throughput

  • High Throughput

Additional Notes

  • figure 1, table S2,S3.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
KIAA0368 VPS11
Co-fractionation
Co-fractionation

Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.

Low-BioGRID
683372

Curated By

  • BioGRID