BAIT
KIAA0368
ECM29, RP11-386D8.2
KIAA0368
GO Process (1)
GO Function (0)
GO Component (12)
Gene Ontology Biological Process
Gene Ontology Cellular Component
Homo sapiens
PREY
MYO1B
myr1
myosin IB
GO Process (5)
GO Function (5)
GO Component (11)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
A protein interaction network for Ecm29 links the 26 S proteasome to molecular motors and endosomal components.
Ecm29 is a 200-kDa HEAT repeat protein that binds the 26 S proteasome. Genome-wide two-hybrid screens and mass spectrometry have identified molecular motors, endosomal components, and ubiquitin-proteasome factors as Ecm29-interacting proteins. The C-terminal half of human Ecm29 binds myosins and kinesins; its N-terminal region binds the endocytic proteins, Vps11, Rab11-FIP4, and rabaptin. Whereas full-length FLAG-Ecm29, its C-terminal half, and a ... [more]
J. Biol. Chem. Oct. 08, 2010; 285(41);31616-33 [Pubmed: 20682791]
Throughput
- Low Throughput
Additional Notes
- figure 1, table S4.
Curated By
- BioGRID