BAIT
KIAA0368
ECM29, RP11-386D8.2
KIAA0368
GO Process (1)
GO Function (0)
GO Component (12)
Gene Ontology Biological Process
Gene Ontology Cellular Component
Homo sapiens
PREY
KIF1B
CMT2, CMT2A, CMT2A1, HMSNII, KLP, NBLST1, RP4-736L20.1
kinesin family member 1B
GO Process (6)
GO Function (5)
GO Component (5)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
A protein interaction network for Ecm29 links the 26 S proteasome to molecular motors and endosomal components.
Ecm29 is a 200-kDa HEAT repeat protein that binds the 26 S proteasome. Genome-wide two-hybrid screens and mass spectrometry have identified molecular motors, endosomal components, and ubiquitin-proteasome factors as Ecm29-interacting proteins. The C-terminal half of human Ecm29 binds myosins and kinesins; its N-terminal region binds the endocytic proteins, Vps11, Rab11-FIP4, and rabaptin. Whereas full-length FLAG-Ecm29, its C-terminal half, and a ... [more]
J. Biol. Chem. Oct. 08, 2010; 285(41);31616-33 [Pubmed: 20682791]
Throughput
- Low Throughput
Additional Notes
- figure 1, table S4.
Curated By
- BioGRID