Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

SUMO modification modulates the transrepression activity of PLZF.

Chao TT, Chang CC, Shih HM

Small ubiquitin-like modifier (SUMO) modification has recently been shown to associate with transcriptional regulation and nuclear body formation. Here, we show that transcription factor PLZF can be SUMO modified at lysine residue 242, 387 and 396. Converting these three SUMO acceptor Lys to Arg 3KR does not significantly affect PLZF nuclear body formation, which is distinct from the scenario of ... [more]

Biochem. Biophys. Res. Commun. Jun. 29, 2007; 358(2);475-82 [Pubmed: 17498654]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
UBE2I ZBTB16
Biochemical Activity
Biochemical Activity

An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation.

Low-BioGRID
685083
UBE2I ZBTB16
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-BioGRID
2708041

Curated By

  • BioGRID