PPP1CC
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
CDK2
Gene Ontology Biological Process
- G1/S transition of mitotic cell cycle [IDA]
- cellular response to insulin stimulus [ISO]
- histone phosphorylation [ISO]
- positive regulation of DNA-dependent DNA replication initiation [IGI]
- positive regulation of cell proliferation [ISO]
- positive regulation of transcription, DNA-templated [IGI]
- potassium ion transport [IGI]
- protein phosphorylation [IDA]
- regulation of cell cycle [IDA, ISO]
- regulation of gene silencing [ISO]
- response to cAMP [ISO]
- response to electrical stimulus [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- Cajal body [ISO]
- X chromosome [IDA]
- Y chromosome [IDA]
- chromosome, telomeric region [IDA]
- condensed chromosome [IDA]
- cyclin-dependent protein kinase holoenzyme complex [IPI, ISO]
- cytoplasm [ISO]
- cytosol [ISO]
- endosome [ISO]
- intracellular membrane-bounded organelle [ISO]
- nucleus [IDA, ISO]
- transcription factor complex [IDA]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
A limited screen for protein interactions reveals new roles for protein phosphatase 1 in cell cycle control and apoptosis.
Protein phosphatase 1 (PP1) catalytic subunits typically combine with other proteins that modulate their activity, direct them to distinct substrates, or serve as substrates for PP1. More than 50 PP1-interacting proteins (PIPs) have been identified so far. Given there are approximately 10 000 phosphoproteins in mammals, many PIPs remain to be discovered. We have used arrays containing 100 carefully selected ... [more]
Throughput
- High Throughput
Curated By
- BioGRID