An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

RNF43 interacts with NEDL1 and regulates p53-mediated transcription.

Shinada K, Tsukiyama T, Sho T, Okumura F, Asaka M, Hatakeyama S

The ubiquitin-proteasomal system plays a crucial role in oncogenesis in colorectal tissues. Recent studies have shown that stability of Î²-catenin, which functions as an oncogene for colorectal cancer, is regulated by ubiquitin-mediated degradation. It has been reported that a putative E3 ubiquitin ligase, RNF43, is highly expressed in human colorectal carcinoma and that RNF43 promotes cell growth. However, the involvement ... [more]

Biochem. Biophys. Res. Commun. Jan. 07, 2011; 404(1);143-7 [Pubmed: 21108931]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
RNF43 HECW1	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Shinada K (2011)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

RNF43

Gene Ontology Biological Process

Gene Ontology Molecular Function

frizzled binding [IPI]protein binding [IPI]ubiquitin-protein transferase activity [IDA]

Gene Ontology Cellular Component

HECW1