Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

RhoA binds to the amino terminus of MEKK1 and regulates its kinase activity.

Gallagher ED, Gutowski S, Sternweis PC, Cobb MH

MEKK1 is a mitogen-activated protein kinase kinase kinase (MAP3K) that can regulate the c-Jun amino-terminal kinase (JNK) MAP kinase cascade. MEKK1 is comprised of a kinase domain and a long amino-terminal regulatory domain. This amino-terminal domain has a scaffold function in that it can assemble modules of the JNK and ERK MAP kinase cascades. Recently, we have demonstrated that MEKK1 ... [more]

J. Biol. Chem. Jan. 16, 2004; 279(3);1872-7 [Pubmed: 14581471]

Throughput

  • Low Throughput

Additional Notes

  • table 1.Mekk1 1-719 as bait.

Curated By

  • BioGRID