An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Interaction proteomics identify NEURL4 and the HECT E3 ligase HERC2 as novel modulators of centrosome architecture.

Al-Hakim AK, Bashkurov M, Gingras AC, Durocher D, Pelletier L

Centrosomes are composed of a centriole pair surrounded by an intricate proteinaceous matrix referred to as pericentriolar material. Although the mechanisms underpinning the control of centriole duplication are now well understood, we know relatively little about the control of centrosome size and shape. Here we used interaction proteomics to identify the E3 ligase HERC2 and the neuralized homologue NEURL4 as ... [more]

Mol. Cell Proteomics Jun. 01, 2012; 11(6);M111.014233 [Pubmed: 22261722]

Throughput

High Throughput

Additional Notes

table 1, S1.

Curated By

BioGRID

Interaction Summary

CCP110

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]

Gene Ontology Cellular Component

GPI