An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Structural and functional analysis of tomosyn identifies domains important in exocytotic regulation.

Williams AL, Bielopolski N, Meroz D, Lam AD, Passmore DR, Ben-Tal N, Ernst SA, Ashery U, Stuenkel EL

Tomosyn is a 130-kDa cytosolic R-SNARE protein that associates with Q-SNAREs and reduces exocytotic activity. Two paralogous genes, tomosyn-1 and -2, occur in mammals and produce seven different isoforms via alternative splicing. Here, we map the structural differences between the yeast homologue of m-tomosyn-1, Sro7, and tomosyn genes/isoforms to identify domains critical to the regulation of exocytotic activity to tomosyn ... [more]

J. Biol. Chem. Apr. 22, 2011; 286(16);14542-53 [Pubmed: 21330375]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

STXBP5

Gene Ontology Biological Process

Gene Ontology Molecular Function

syntaxin binding [ISO]syntaxin-1 binding [IDA, ISO]

Gene Ontology Cellular Component

SUMO3