An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Distinct chaperone mechanisms can delay the formation of aggresomes by the myopathy-causing R120G alphaB-crystallin mutant.

Chavez Zobel AT, Loranger A, Marceau N, Theriault JR, Lambert H, Landry J

A familial form of desmin-related myopathy (DRM) is associated with a missense mutation (R120G) in alphaB-crystallin (alphaB) and is characterized by intracellular desmin aggregation. Because alphaB is a molecular chaperone that participates in the assembly of desmin filaments, it has been suggested that the desmin aggregation might be due to the loss of alphaB function. We report here that alphaBR120G ... [more]

Hum. Mol. Genet. Jul. 01, 2003; 12(13);1609-20 [Pubmed: 12812987]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

HSPA4

Gene Ontology Biological Process

Gene Ontology Molecular Function

ATP binding [NAS]

Gene Ontology Cellular Component

CRYAB