SMAD3
Gene Ontology Biological Process
- SMAD protein complex assembly [IDA]
- activation of cysteine-type endopeptidase activity involved in apoptotic process [IMP]
- activin receptor signaling pathway [IMP]
- cell cycle arrest [IMP]
- cell-cell junction organization [IMP]
- evasion or tolerance of host defenses by virus [IDA]
- extrinsic apoptotic signaling pathway [IMP]
- gene expression [TAS]
- immune response [IMP]
- intracellular signal transduction [IDA]
- negative regulation of cell growth [IDA]
- negative regulation of mitotic cell cycle [IMP]
- negative regulation of protein catabolic process [IMP]
- negative regulation of protein phosphorylation [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IDA, IMP, TAS]
- negative regulation of transforming growth factor beta receptor signaling pathway [TAS]
- nodal signaling pathway [IMP]
- positive regulation of canonical Wnt signaling pathway [IMP]
- positive regulation of catenin import into nucleus [IMP]
- positive regulation of epithelial to mesenchymal transition [IDA, IMP]
- positive regulation of gene expression [IDA]
- positive regulation of transcription factor import into nucleus [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IDA, IMP, TAS]
- positive regulation of transcription, DNA-templated [IDA]
- primary miRNA processing [TAS]
- protein stabilization [IMP]
- regulation of transcription from RNA polymerase II promoter [IDA]
- regulation of transforming growth factor beta receptor signaling pathway [IMP]
- regulation of transforming growth factor beta2 production [IMP]
- response to hypoxia [IMP]
- transcription initiation from RNA polymerase II promoter [TAS]
- transcription, DNA-templated [TAS]
- transforming growth factor beta receptor signaling pathway [IDA, TAS]
- transport [IDA]
- wound healing [TAS]
Gene Ontology Molecular Function- R-SMAD binding [IPI]
- RNA polymerase II activating transcription factor binding [IPI]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity [IDA]
- bHLH transcription factor binding [IPI]
- beta-catenin binding [IPI]
- co-SMAD binding [IPI]
- core promoter proximal region sequence-specific DNA binding [IDA]
- enhancer binding [IC]
- identical protein binding [IPI]
- phosphatase binding [IPI]
- protein binding [IPI]
- protein binding transcription factor activity [IDA]
- protein homodimerization activity [IPI]
- protein kinase binding [IPI]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription factor binding [IPI]
- transcription regulatory region DNA binding [IDA]
- transforming growth factor beta receptor binding [IPI]
- transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity [IDA]
- ubiquitin binding [IDA]
- ubiquitin protein ligase binding [IPI]
- zinc ion binding [IDA]
- R-SMAD binding [IPI]
- RNA polymerase II activating transcription factor binding [IPI]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity [IDA]
- bHLH transcription factor binding [IPI]
- beta-catenin binding [IPI]
- co-SMAD binding [IPI]
- core promoter proximal region sequence-specific DNA binding [IDA]
- enhancer binding [IC]
- identical protein binding [IPI]
- phosphatase binding [IPI]
- protein binding [IPI]
- protein binding transcription factor activity [IDA]
- protein homodimerization activity [IPI]
- protein kinase binding [IPI]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription factor binding [IPI]
- transcription regulatory region DNA binding [IDA]
- transforming growth factor beta receptor binding [IPI]
- transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity [IDA]
- ubiquitin binding [IDA]
- ubiquitin protein ligase binding [IPI]
- zinc ion binding [IDA]
Gene Ontology Cellular Component
USP15
Gene Ontology Biological Process
- BMP signaling pathway [IDA]
- histone H2B conserved C-terminal lysine deubiquitination [IDA]
- monoubiquitinated protein deubiquitination [IDA]
- negative regulation of transforming growth factor beta receptor signaling pathway [TAS]
- pathway-restricted SMAD protein phosphorylation [IMP]
- proteasome-mediated ubiquitin-dependent protein catabolic process [IBA]
- protein deubiquitination [IDA]
- regulation of proteasomal protein catabolic process [IBA]
- transforming growth factor beta receptor signaling pathway [IDA, IMP, TAS]
Gene Ontology Molecular Function
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
USP15 stabilizes TGF-β receptor I and promotes oncogenesis through the activation of TGF-β signaling in glioblastoma.
In advanced cancer, including glioblastoma, the transforming growth factor β (TGF-β) pathway acts as an oncogenic factor and is considered to be a therapeutic target. Using a functional RNAi screen, we identified the deubiquitinating enzyme ubiquitin-specific peptidase 15 (USP15) as a key component of the TGF-β signaling pathway. USP15 binds to the SMAD7-SMAD specific E3 ubiquitin protein ligase 2 (SMURF2) ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| SMAD3 USP15 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| USP15 SMAD3 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| USP15 SMAD3 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | 1510765 | |
| USP15 SMAD3 | Biochemical Activity Biochemical Activity An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation. | Low | - | BioGRID | 611708 | |
| SMAD3 USP15 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - |
Curated By
- BioGRID