HIPK1
Gene Ontology Biological Process
- anterior/posterior pattern specification [IGI]
- embryonic camera-type eye morphogenesis [IMP]
- embryonic retina morphogenesis in camera-type eye [IMP]
- endothelial cell apoptotic process [ISO]
- extrinsic apoptotic signaling pathway [ISO]
- intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator [IGI]
- iris morphogenesis [IMP]
- lens induction in camera-type eye [IMP]
- negative regulation of transcription from RNA polymerase II promoter [ISS]
- neuron differentiation [IMP]
- positive regulation of DNA binding [ISS]
- positive regulation of cell proliferation [IGI]
- protein phosphorylation [ISS]
- regulation of tumor necrosis factor-mediated signaling pathway [ISO]
- retina layer formation [IMP]
- smoothened signaling pathway [IGI]
Gene Ontology Molecular Function
MDM2
Gene Ontology Biological Process
- DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest [ISO]
- establishment of protein localization [ISO]
- negative regulation of DNA damage response, signal transduction by p53 class mediator [ISO]
- negative regulation of apoptotic process [ISO]
- negative regulation of cell cycle arrest [ISO]
- negative regulation of cysteine-type endopeptidase activity involved in apoptotic process [ISO]
- negative regulation of gene expression [ISO]
- negative regulation of protein processing [ISO]
- negative regulation of transcription from RNA polymerase II promoter [ISO]
- negative regulation of transcription, DNA-templated [ISO]
- peptidyl-lysine modification [ISO]
- positive regulation of cell cycle [IGI]
- positive regulation of gene expression [ISO]
- positive regulation of mitotic cell cycle [ISO]
- positive regulation of proteasomal ubiquitin-dependent protein catabolic process [ISO]
- positive regulation of protein export from nucleus [ISO]
- protein catabolic process [IDA]
- protein complex assembly [ISO]
- protein destabilization [ISO]
- protein localization to nucleus [ISO]
- protein ubiquitination [IDA, ISO]
- protein ubiquitination involved in ubiquitin-dependent protein catabolic process [ISO]
- regulation of protein catabolic process [ISO]
- traversing start control point of mitotic cell cycle [IDA]
Gene Ontology Molecular Function
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Mdm2 associates with Ras effector NORE1 to induce the degradation of oncoprotein HIPK1.
The Ras effector NORE1 is frequently silenced in primary adenocarcinomas, although the significance of this silencing for tumorigenesis is unclear. Here we show that NORE1 induces polyubiquitination and proteasomal degradation of oncoprotein HIPK1 by facilitating its interaction with the Mdm2 E3 ubiquitin ligase. Endogenous HIPK1 is stabilized in Nore1-deficient mouse embryonic fibroblasts, and depletion of HIPK1 in NORE1-silenced lung adenocarcinoma ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID