TFAP4
Gene Ontology Biological Process
- DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator [IDA]
- negative regulation by host of viral transcription [IDA, NAS]
- negative regulation of DNA binding [IDA]
- negative regulation of cell cycle arrest [IMP]
- negative regulation of cell proliferation [IMP]
- negative regulation of cyclin-dependent protein serine/threonine kinase activity [IDA]
- negative regulation of transcription, DNA-templated [IDA, IMP]
- positive regulation by host of viral transcription [IDA]
- positive regulation of apoptotic process [IDA]
- positive regulation of transcription, DNA-templated [IMP]
- protein complex assembly [IDA]
- regulation of transcription from RNA polymerase II promoter [IBA]
- transcription from RNA polymerase II promoter [IDA]
Gene Ontology Molecular Function- DNA binding [IDA]
- E-box binding [IDA]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [IDA]
- histone deacetylase binding [IPI]
- protein binding [IPI]
- protein heterodimerization activity [IMP]
- protein homodimerization activity [IPI]
- sequence-specific DNA binding [IDA]
- transcription coactivator activity [IDA]
- transcription regulatory region DNA binding [IDA]
- DNA binding [IDA]
- E-box binding [IDA]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [IDA]
- histone deacetylase binding [IPI]
- protein binding [IPI]
- protein heterodimerization activity [IMP]
- protein homodimerization activity [IPI]
- sequence-specific DNA binding [IDA]
- transcription coactivator activity [IDA]
- transcription regulatory region DNA binding [IDA]
Gene Ontology Cellular Component
CTBP1
Gene Ontology Biological Process
- chromatin organization involved in regulation of transcription [IMP]
- negative regulation of cell proliferation [TAS]
- negative regulation of histone H4 acetylation [IMP]
- negative regulation of histone acetylation [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IMP]
- negative regulation of transcription, DNA-templated [ISS]
- positive regulation of histone deacetylation [IMP]
- protein phosphorylation [TAS]
- regulation of cell cycle [IMP]
- viral genome replication [TAS]
- white fat cell differentiation [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Complementary quantitative proteomics reveals that transcription factor AP-4 mediates E-box-dependent complex formation for transcriptional repression of HDM2.
Transcription factor activating enhancer-binding protein 4 (AP-4) is a basic helix-loop-helix protein that binds to E-box elements. AP-4 has received increasing attention for its regulatory role in cell growth and development, including transcriptional repression of the human homolog of murine double minute 2 (HDM2), an important oncoprotein controlling cell growth and survival, by an unknown mechanism. Here we demonstrate that ... [more]
Throughput
- High Throughput
Additional Notes
- table 1. potential AP-4-interacting proteins differentially bound to the HDM2-P2 promoter E-box sequence.
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| TFAP4 CTBP1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | High | - | BioGRID | 685790 |
Curated By
- BioGRID