TFAP4
Gene Ontology Biological Process
- DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator [IDA]
- negative regulation by host of viral transcription [IDA, NAS]
- negative regulation of DNA binding [IDA]
- negative regulation of cell cycle arrest [IMP]
- negative regulation of cell proliferation [IMP]
- negative regulation of cyclin-dependent protein serine/threonine kinase activity [IDA]
- negative regulation of transcription, DNA-templated [IDA, IMP]
- positive regulation by host of viral transcription [IDA]
- positive regulation of apoptotic process [IDA]
- positive regulation of transcription, DNA-templated [IMP]
- protein complex assembly [IDA]
- regulation of transcription from RNA polymerase II promoter [IBA]
- transcription from RNA polymerase II promoter [IDA]
Gene Ontology Molecular Function- DNA binding [IDA]
- E-box binding [IDA]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [IDA]
- histone deacetylase binding [IPI]
- protein binding [IPI]
- protein heterodimerization activity [IMP]
- protein homodimerization activity [IPI]
- sequence-specific DNA binding [IDA]
- transcription coactivator activity [IDA]
- transcription regulatory region DNA binding [IDA]
- DNA binding [IDA]
- E-box binding [IDA]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [IDA]
- histone deacetylase binding [IPI]
- protein binding [IPI]
- protein heterodimerization activity [IMP]
- protein homodimerization activity [IPI]
- sequence-specific DNA binding [IDA]
- transcription coactivator activity [IDA]
- transcription regulatory region DNA binding [IDA]
Gene Ontology Cellular Component
CTCF
Gene Ontology Biological Process
- negative regulation of transcription from RNA polymerase II promoter [IDA]
- negative regulation of transcription, DNA-templated [IDA]
- nucleosome positioning [IDA]
- positive regulation of gene expression [IMP]
- positive regulation of transcription, DNA-templated [IDA, IMP]
- regulation of centromeric sister chromatid cohesion [NAS]
- regulation of gene expression, epigenetic [IMP]
- regulation of molecular function, epigenetic [IMP]
Gene Ontology Molecular Function- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [IDA]
- chromatin insulator sequence binding [IDA]
- protein binding [IPI]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription corepressor activity [TAS]
- transcription regulatory region DNA binding [IDA]
- zinc ion binding [TAS]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [IDA]
- chromatin insulator sequence binding [IDA]
- protein binding [IPI]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription corepressor activity [TAS]
- transcription regulatory region DNA binding [IDA]
- zinc ion binding [TAS]
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Complementary quantitative proteomics reveals that transcription factor AP-4 mediates E-box-dependent complex formation for transcriptional repression of HDM2.
Transcription factor activating enhancer-binding protein 4 (AP-4) is a basic helix-loop-helix protein that binds to E-box elements. AP-4 has received increasing attention for its regulatory role in cell growth and development, including transcriptional repression of the human homolog of murine double minute 2 (HDM2), an important oncoprotein controlling cell growth and survival, by an unknown mechanism. Here we demonstrate that ... [more]
Throughput
- High Throughput
Additional Notes
- figure 3.
- figure 5.
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| TFAP4 CTCF | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | 685748 |
Curated By
- BioGRID