An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Structural dissection of a gating mechanism preventing misactivation of ubiquitin by NEDD8's E1.

Souphron J, Waddell MB, Paydar A, Tokgoez-Gromley Z, Roussel MF, Schulman BA

Post-translational covalent modification by ubiquitin and ubiquitin-like proteins (UBLs) is a major eukaryotic mechanism for regulating protein function. In general, each UBL has its own E1 that serves as the entry point for a cascade. The E1 first binds the UBL and catalyzes adenylation of the UBL's C-terminus, prior to promoting UBL transfer to a downstream E2. Ubiquitin's Arg 72, ... [more]

Biochemistry Aug. 26, 2008; 47(34);8961-9 [Pubmed: 18652489]

Throughput

Low Throughput

Additional Notes

APPBP1-UBA3 Arg190Gln and Arg190Ala mutants can transfer NEDD8Ala72Ar to Ubc12

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
UBE2M NAE1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Cho NH (2022)	High	-	BioGRID	3374471
UBE2M NAE1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Ewing RM (2007)	High	0.463	BioGRID	241717
UBE2M NAE1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Huttlin EL (2017)	High	1	BioGRID	2225117
UBE2M NAE1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Huttlin EL (2021/pre-pub)	High	0.998	BioGRID	3284878
UBE2M NAE1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Huttlin EL (2021)	High	1	BioGRID	3093702
UBE2M NAE1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Scott DC (2017)	High	-	BioGRID	-
NAE1 UBE2M	Biochemical Activity Biochemical Activity An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation.	Huang DT (2009)	Low	-	BioGRID	1059805
NAE1 UBE2M	Biochemical Activity Biochemical Activity An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation.	Xiong C (2021)	Low	-	BioGRID	3217538
NAE1 UBE2M	Co-crystal Structure Co-crystal Structure Interaction directly demonstrated at the atomic level by X-ray crystallography. Also used for NMR or Electron Microscopy (EM) structures. If there is no obvious bait-hit directionality to the interaction involving 3 or more proteins, then the co-crystallized proteins should be listed as a complex.	Huang DT (2007)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

NAE1

Gene Ontology Biological Process

Gene Ontology Molecular Function

NEDD8 activating enzyme activity [IDA]protein binding [IPI]protein heterodimerization activity [IPI]ubiquitin protein ligase binding [IPI]

Gene Ontology Cellular Component

UBE2M

Gene Ontology Biological Process

Gene Ontology Molecular Function

NEDD8 transferase activity [IDA]protein binding [IPI]ribosomal S6-glutamic acid ligase activity [IDA]ubiquitin protein ligase activity [IBA]ubiquitin protein ligase binding [IBA]ubiquitin-protein transferase activity [IMP, TAS]