BAIT
CUL4B
CUL-4B, MRXHF2, MRXS15, MRXSC, SFM2
cullin 4B
GO Process (3)
GO Function (2)
GO Component (5)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
JUP
ARVD12, CTNNG, DP3, DPIII, PDGB, PKGB
junction plakoglobin
GO Process (20)
GO Function (9)
GO Component (17)
Gene Ontology Biological Process
- adherens junction organization [TAS]
- bundle of His cell to Purkinje myocyte communication [IMP]
- cell junction assembly [TAS]
- cell migration [IMP]
- cell-cell junction organization [TAS]
- cellular response to indole-3-methanol [IDA]
- cytoskeletal anchoring at plasma membrane [NAS]
- desmosome assembly [IDA, IMP]
- detection of mechanical stimulus [IDA]
- endothelial cell-cell adhesion [ISS]
- establishment of protein localization to plasma membrane [IMP]
- positive regulation of canonical Wnt signaling pathway [IC]
- positive regulation of protein import into nucleus [IDA]
- positive regulation of sequence-specific DNA binding transcription factor activity [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IBA]
- regulation of cell fate specification [IBA]
- regulation of cell proliferation [IDA]
- regulation of heart rate by cardiac conduction [IMP]
- single organismal cell-cell adhesion [IDA, IMP]
- ventricular cardiac muscle cell action potential [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- catenin complex [IDA]
- cell-cell adherens junction [IDA]
- cell-cell junction [IDA]
- cytoplasm [IMP]
- cytoplasmic side of plasma membrane [ISS]
- cytoskeleton [ISS]
- cytosol [ISS]
- desmosome [IDA]
- extracellular vesicular exosome [IDA]
- focal adhesion [IDA]
- gamma-catenin-TCF7L2 complex [IDA]
- hemidesmosome [ISS]
- intercalated disc [IDA]
- nucleus [IMP]
- plasma membrane [IDA, TAS]
- protein-DNA complex [IDA]
- zonula adherens [ISS]
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dynamics of cullin-RING ubiquitin ligase network revealed by systematic quantitative proteomics.
Dynamic reorganization of signaling systems frequently accompanies pathway perturbations, yet quantitative studies of network remodeling by pathway stimuli are lacking. Here, we report the development of a quantitative proteomics platform centered on multiplex absolute quantification (AQUA) technology to elucidate the architecture of the cullin-RING ubiquitin ligase (CRL) network and to evaluate current models of dynamic CRL remodeling. Current models suggest ... [more]
Cell Dec. 10, 2010; 143(6);951-65 [Pubmed: 21145461]
Throughput
- High Throughput
Ontology Terms
- cell line: hek-293t cell (BTO:0002181)
Additional Notes
- All data was filtered to a 1% false discovery rate (peptide level) prior to analysis using CompPASS to identify high confidence candidate interacting proteins
- TAP-tagged Cul4B
- exogenous expression of bait
Curated By
- BioGRID