CUL5
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
CLTC
Gene Ontology Biological Process
- antigen processing and presentation of exogenous peptide antigen via MHC class II [TAS]
- intracellular protein transport [NAS]
- membrane organization [TAS]
- mitotic nuclear division [IMP]
- negative regulation of hyaluronan biosynthetic process [IDA, IMP]
- negative regulation of protein localization to plasma membrane [IMP]
- osteoblast differentiation [IDA]
- post-Golgi vesicle-mediated transport [TAS]
- receptor internalization [IMP]
- receptor-mediated endocytosis [IMP]
- transferrin transport [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- clathrin coat [NAS]
- clathrin complex [IDA]
- clathrin-coated endocytic vesicle membrane [TAS]
- clathrin-coated vesicle [IDA]
- cytoplasm [IDA]
- cytosol [TAS]
- extracellular vesicular exosome [IDA]
- focal adhesion [IDA]
- intracellular membrane-bounded organelle [IDA]
- membrane [IDA]
- plasma membrane [TAS]
- protein complex [IDA]
- spindle [IDA]
- trans-Golgi network membrane [TAS]
- vesicle [IDA]
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dynamics of cullin-RING ubiquitin ligase network revealed by systematic quantitative proteomics.
Dynamic reorganization of signaling systems frequently accompanies pathway perturbations, yet quantitative studies of network remodeling by pathway stimuli are lacking. Here, we report the development of a quantitative proteomics platform centered on multiplex absolute quantification (AQUA) technology to elucidate the architecture of the cullin-RING ubiquitin ligase (CRL) network and to evaluate current models of dynamic CRL remodeling. Current models suggest ... [more]
Throughput
- High Throughput
Ontology Terms
- cell line: hek-293t cell (BTO:0002181)
Additional Notes
- All data was filtered to a 1% false discovery rate (peptide level) prior to analysis using CompPASS to identify high confidence candidate interacting proteins
- TAP-tagged Cul5
- exogenous expression of bait
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| CLTC CUL5 | Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex. | High | - | BioGRID | 3448234 |
Curated By
- BioGRID