BAIT

CDC73

L000002792, YLR418C

Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress; human homologue, parafibromin, is a tumour suppressor linked to breast, renal and gastric cancers

GO Process (11)

GO Function (5)

GO Component (3)

Gene Ontology Molecular Function

RNA polymerase II C-terminal domain phosphoserine binding [IDA]
RNA polymerase II core binding [IPI]
RNA polymerase II transcription factor binding transcription factor activity [IPI]
TFIIF-class binding transcription factor activity [IMP, IPI]
chromatin binding [IDA]

Gene Ontology Cellular Component

Cdc73/Paf1 complex [IPI]

nucleus [IDA]

transcriptionally active chromatin [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

SPT16

CDC68, SSF1, chromatin-remodeling protein SPT16, L000002038, YGL207W

Subunit of the heterodimeric FACT complex (Spt16p-Pob3p); FACT associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; SPT16 specifically required for diauxic shift-induced H2B deposition onto rDNA genes; some mutations cause reduced nucleosome occupancy over highly transcribed regions of the yeast genome

GO Process (7)

GO Function (3)

GO Component (5)

Gene Ontology Biological Process

DNA replication-independent nucleosome organization [IDA]

DNA-dependent DNA replication [IPI]

chromatin organization involved in regulation of transcription [IMP]

nucleosome assembly [IDA]

positive regulation of RNA polymerase II transcriptional preinitiation complex assembly [IDA]

positive regulation of transcription initiation from RNA polymerase II promoter [IGI, IMP]

transcription elongation from RNA polymerase II promoter [IPI]

Gene Ontology Molecular Function

chromatin binding [IDA]
histone binding [IDA]
nucleosome binding [IDA]

Gene Ontology Cellular Component

FACT complex [IGI, IPI]

alpha DNA polymerase:primase complex [IDA]

nuclear chromatin [IDA]

replication fork protection complex [IDA]

transcription elongation factor complex [IPI]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

High-definition macromolecular composition of yeast RNA-processing complexes.

Krogan NJ, Peng WT, Cagney G, Robinson MD, Haw R, Zhong G, Guo X, Zhang X, Canadien V, Richards DP, Beattie BK, Lalev A, Zhang W, Davierwala AP, Mnaimneh S, Starostine A, Tikuisis AP, Grigull J, Datta N, Bray JE, Hughes TR, Emili A, Greenblatt JF

A remarkably large collection of evolutionarily conserved proteins has been implicated in processing of noncoding RNAs and biogenesis of ribonucleoproteins. To better define the physical and functional relationships among these proteins and their cognate RNAs, we performed 165 highly stringent affinity purifications of known or predicted RNA-related proteins from Saccharomyces cerevisiae. We systematically identified and estimated the relative abundance of ... [more]

Mol. Cell Jan. 30, 2004; 13(2);225-39 [Pubmed: 14759368]

Throughput

High Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
SPT16 CDC73	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Bedard LG (2016)	High	-	BioGRID	1537483
SPT16 CDC73	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Foltman M (2013)	Low	-	BioGRID	1277109
SPT16 CDC73	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Gavin AC (2002)	High	-	BioGRID	-
SPT16 CDC73	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Gavin AC (2006)	High	-	BioGRID	-
CDC73 SPT16	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Krogan NJ (2002)	Low	-	BioGRID	-
SPT16 CDC73	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Krogan NJ (2002)	Low	-	BioGRID	-
SPT16 CDC73	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Krogan NJ (2004)	High	-	BioGRID	-
SPT16 CDC73	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Krogan NJ (2006)	High	-	BioGRID	-
SPT16 CDC73	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Leng H (2021)	High	-	BioGRID	-
SPT16 CDC73	Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.	Stevens JR (2011)	Low	-	BioGRID	574048

Curated By

BioGRID

Interaction Summary

CDC73

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase II C-terminal domain phosphoserine binding [IDA]RNA polymerase II core binding [IPI]RNA polymerase II transcription factor binding transcription factor activity [IPI]TFIIF-class binding transcription factor activity [IMP, IPI]chromatin binding [IDA]

Gene Ontology Cellular Component

SPT16

Gene Ontology Biological Process

Gene Ontology Molecular Function

chromatin binding [IDA]histone binding [IDA]nucleosome binding [IDA]

Gene Ontology Cellular Component

Affinity Capture-MS

Publication

High-definition macromolecular composition of yeast RNA-processing complexes.

Throughput

Related interactions

Curated By

RNA polymerase II C-terminal domain phosphoserine binding [IDA]
RNA polymerase II core binding [IPI]
RNA polymerase II transcription factor binding transcription factor activity [IPI]
TFIIF-class binding transcription factor activity [IMP, IPI]
chromatin binding [IDA]

chromatin binding [IDA]
histone binding [IDA]
nucleosome binding [IDA]