An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Dynamics of cullin-RING ubiquitin ligase network revealed by systematic quantitative proteomics.

Bennett EJ, Rush J, Gygi SP, Harper JW

Dynamic reorganization of signaling systems frequently accompanies pathway perturbations, yet quantitative studies of network remodeling by pathway stimuli are lacking. Here, we report the development of a quantitative proteomics platform centered on multiplex absolute quantification (AQUA) technology to elucidate the architecture of the cullin-RING ubiquitin ligase (CRL) network and to evaluate current models of dynamic CRL remodeling. Current models suggest ... [more]

Cell Dec. 10, 2010; 143(6);951-65 [Pubmed: 21145461]

Throughput

High Throughput

Ontology Terms

cell line: hek-293t cell (BTO:0002181)

Additional Notes

All data was filtered to a 1% false discovery rate (peptide level) prior to analysis using CompPASS to identify high confidence candidate interacting proteins
TAP-tagged Cul2
exogenous expression of bait

Curated By

BioGRID

Interaction Summary

CUL2

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]ubiquitin protein ligase binding [IDA]

Gene Ontology Cellular Component

DNAJA1

Gene Ontology Biological Process

Gene Ontology Molecular Function

G-protein coupled receptor binding [IPI]Hsp70 protein binding [IDA]chaperone binding [IDA, IPI]low-density lipoprotein particle receptor binding [IDA]protein binding [IPI]ubiquitin protein ligase binding [IPI]unfolded protein binding [IBA]