An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Dynamics of cullin-RING ubiquitin ligase network revealed by systematic quantitative proteomics.

Bennett EJ, Rush J, Gygi SP, Harper JW

Dynamic reorganization of signaling systems frequently accompanies pathway perturbations, yet quantitative studies of network remodeling by pathway stimuli are lacking. Here, we report the development of a quantitative proteomics platform centered on multiplex absolute quantification (AQUA) technology to elucidate the architecture of the cullin-RING ubiquitin ligase (CRL) network and to evaluate current models of dynamic CRL remodeling. Current models suggest ... [more]

Cell Dec. 10, 2010; 143(6);951-65 [Pubmed: 21145461]

Throughput

High Throughput

Ontology Terms

cell line: hek-293t cell (BTO:0002181)

Additional Notes

All data was filtered to a 1% false discovery rate (peptide level) prior to analysis using CompPASS to identify high confidence candidate interacting proteins
TAP-tagged COPS5
exogenous expression of bait

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
COPS5 KCTD10	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Horlbeck MA (2018)	High	-3.3107	BioGRID	2456814

Curated By

BioGRID

Interaction Summary

COPS5

Gene Ontology Biological Process

Gene Ontology Molecular Function

metallopeptidase activity [IMP]protein binding [IPI]transcription coactivator activity [TAS]translation initiation factor activity [TAS]ubiquitin-specific protease activity [IDA]

Gene Ontology Cellular Component

KCTD10