BAIT
CAND1
TIP120, TIP120A
cullin-associated and neddylation-dissociated 1
GO Process (4)
GO Function (1)
GO Component (7)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
EIF2AK2
EIF2AK1, PKR, PPP1R83, PRKR
eukaryotic translation initiation factor 2-alpha kinase 2
GO Process (22)
GO Function (7)
GO Component (4)
Gene Ontology Biological Process
- activation of MAPKK activity [IMP]
- evasion or tolerance by virus of host immune response [TAS]
- modulation by virus of host morphology or physiology [TAS]
- modulation by virus of host process [TAS]
- negative regulation of cell proliferation [TAS]
- negative regulation of osteoblast proliferation [IMP]
- negative regulation of translation [IDA, IMP]
- negative regulation of viral genome replication [IMP]
- positive regulation of NF-kappaB transcription factor activity [IDA]
- positive regulation of NIK/NF-kappaB signaling [ISS]
- positive regulation of chemokine production [ISS]
- positive regulation of cytokine production [ISS]
- positive regulation of stress-activated MAPK cascade [ISS]
- protein autophosphorylation [IDA, IMP]
- protein phosphorylation [IDA]
- regulation of NLRP3 inflammasome complex assembly [ISS]
- regulation of hematopoietic progenitor cell differentiation [ISS]
- regulation of hematopoietic stem cell differentiation [ISS]
- regulation of hematopoietic stem cell proliferation [ISS]
- response to interferon-alpha [IDA]
- response to virus [IMP]
- viral life cycle [TAS]
Gene Ontology Molecular Function
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dynamics of cullin-RING ubiquitin ligase network revealed by systematic quantitative proteomics.
Dynamic reorganization of signaling systems frequently accompanies pathway perturbations, yet quantitative studies of network remodeling by pathway stimuli are lacking. Here, we report the development of a quantitative proteomics platform centered on multiplex absolute quantification (AQUA) technology to elucidate the architecture of the cullin-RING ubiquitin ligase (CRL) network and to evaluate current models of dynamic CRL remodeling. Current models suggest ... [more]
Cell Dec. 10, 2010; 143(6);951-65 [Pubmed: 21145461]
Throughput
- High Throughput
Ontology Terms
- cell line: hek-293t cell (BTO:0002181)
Additional Notes
- All data was filtered to a 1% false discovery rate (peptide level) prior to analysis using CompPASS to identify high confidence candidate interacting proteins
- TAP-tagged CAND1
- exogenous expression of bait
Curated By
- BioGRID