BAIT
CAND1
TIP120, TIP120A
cullin-associated and neddylation-dissociated 1
GO Process (4)
GO Function (1)
GO Component (7)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
PDCD6
ALG-2, ALG2, PEF1B
programmed cell death 6
GO Process (13)
GO Function (7)
GO Component (6)
Gene Ontology Biological Process
- apoptotic signaling pathway [TAS]
- cellular response to heat [IDA]
- intracellular protein transport [IDA]
- negative regulation of TOR signaling [IDA]
- negative regulation of protein kinase B signaling [IDA]
- negative regulation of vascular endothelial growth factor receptor signaling pathway [IDA]
- positive regulation of angiogenesis [IDA]
- positive regulation of cysteine-type endopeptidase activity involved in apoptotic process [IDA]
- positive regulation of endothelial cell migration [IDA]
- positive regulation of endothelial cell proliferation [IDA]
- proteolysis [IBA]
- response to calcium ion [ISS]
- vascular endothelial growth factor receptor-2 signaling pathway [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dynamics of cullin-RING ubiquitin ligase network revealed by systematic quantitative proteomics.
Dynamic reorganization of signaling systems frequently accompanies pathway perturbations, yet quantitative studies of network remodeling by pathway stimuli are lacking. Here, we report the development of a quantitative proteomics platform centered on multiplex absolute quantification (AQUA) technology to elucidate the architecture of the cullin-RING ubiquitin ligase (CRL) network and to evaluate current models of dynamic CRL remodeling. Current models suggest ... [more]
Cell Dec. 10, 2010; 143(6);951-65 [Pubmed: 21145461]
Throughput
- High Throughput
Ontology Terms
- cell line: hek-293t cell (BTO:0002181)
Additional Notes
- All data was filtered to a 1% false discovery rate (peptide level) prior to analysis using CompPASS to identify high confidence candidate interacting proteins
- TAP-tagged CAND1
- exogenous expression of bait
Curated By
- BioGRID