BAIT

SRC

AW259666, pp60c-src, RP23-169M4.1
Rous sarcoma oncogene
GO Process (29)
GO Function (9)
GO Component (6)
Mus musculus
PREY

INPP5D

SHIP, SHIP-1, SHIP1, SIP-145, p150Ship, s-SHIP
inositol polyphosphate-5-phosphatase D
Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Src homology 2 (SH2)-containing 5'-inositol phosphatase localizes to podosomes, and the SH2 domain is implicated in the attenuation of bone resorption in osteoclasts.

Yogo K, Mizutamari M, Mishima K, Takenouchi H, Ishida-Kitagawa N, Sasaki T, Takeya T

c-Src plays an important role in bone resorption by osteoclasts. Here, we show using wild-type and ship(-/-) osteoclasts that Src homology 2 (SH2)-containing 5'-inositol phosphatase (SHIP) appeared to negatively regulate bone resorption activated by c-Src. SHIP was found to localize to podosomes under the influence of c-Src, and the presence of either the amino-terminal region comprising the SH2 domain or ... [more]

Endocrinology Jul. 01, 2006; 147(7);3307-17 [Pubmed: 16601135]

Throughput

  • Low Throughput

Curated By

  • BioGRID