Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Complexes with mixed primary and secondary cellulose synthases are functional in Arabidopsis thaliana plants.

Carroll A, Mansoori N, Li S, Lei L, Vernhettes S, Visser RG, Somerville C, Gu Y, Trindade LM

In higher plants cellulose is synthesized by so called rosette protein complexes with cellulose synthases (CESAs) as catalytic subunits of the complex. The CESAs are divided into two distinct families, three of which are thought to be specialized for the primary cell wall and three for the secondary cell wall. In this article the potential of primary and secondary CESAs ... [more]

Plant Physiol. Aug. 27, 2012; 0(0); [Pubmed: 22926318]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
CESA6 CESA4	PCA PCA A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.	Carroll A (2012)	Low	-	BioGRID	-
CESA4 CESA6	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Carroll A (2012)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

CESA6

Gene Ontology Biological Process

Gene Ontology Molecular Function

cellulose synthase activity [ISS]transferase activity, transferring glycosyl groups [ISS]

Gene Ontology Cellular Component

CESA4