NR3C1
Gene Ontology Biological Process
- cellular response to steroid hormone stimulus [IDA]
- gene expression [TAS]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- regulation of transcription, DNA-templated [IDA]
- signal transduction [TAS]
- transcription from RNA polymerase II promoter [TAS]
- transcription initiation from RNA polymerase II promoter [TAS]
- transcription, DNA-templated [TAS]
Gene Ontology Molecular Function- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- glucocorticoid receptor activity [TAS]
- glucocorticoid-activated RNA polymerase II transcription factor binding transcription factor activity [IDA]
- protein binding [IPI]
- sequence-specific DNA binding transcription factor activity [IDA]
- steroid binding [IDA]
- steroid hormone binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- glucocorticoid receptor activity [TAS]
- glucocorticoid-activated RNA polymerase II transcription factor binding transcription factor activity [IDA]
- protein binding [IPI]
- sequence-specific DNA binding transcription factor activity [IDA]
- steroid binding [IDA]
- steroid hormone binding [IDA]
Gene Ontology Cellular Component
UBE2L3
Gene Ontology Biological Process
- cell cycle phase transition [IMP]
- cell proliferation [IMP]
- cellular protein modification process [TAS]
- cellular response to glucocorticoid stimulus [IDA]
- cellular response to steroid hormone stimulus [IMP]
- positive regulation of protein ubiquitination [IGI]
- positive regulation of ubiquitin-protein transferase activity [IGI]
- protein K11-linked ubiquitination [IDA]
- protein polyubiquitination [IDA]
- protein ubiquitination [IDA]
- regulation of transcription, DNA-templated [IDA, IMP]
- ubiquitin-dependent protein catabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
UbcH7 interacts with the glucocorticoid receptor and mediates receptor autoregulation.
Unlike other nuclear receptors, transactivation by the glucocorticoid receptor (GR) is increased by the inhibition of the ubiquitin/proteasome pathway. Here, we demonstrate that the ubiquitin-conjugating enzyme (E2), UbcH7, physically interacts with the GR and, when overexpressed, reduces the ability of the receptor to upregulate gene expression. Chemical inhibition of the 26S proteasome abolished the downregulation effect of overexpressed UbcH7, suggesting ... [more]
Throughput
- Low Throughput
Additional Notes
- Two-hybrid interaction in the presence of RU486.
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| NR3C1 UBE2L3 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID