BAIT

FUS

ALS6, ETM4, FUS1, HNRNPP2, POMP75, TLS
FUS RNA binding protein
GO Process (3)
GO Function (4)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens
PREY

ARHGEF28

RGNEF, RIP2, p190RHOGEF
Rho guanine nucleotide exchange factor (GEF) 28
GO Process (0)
GO Function (0)
GO Component (0)
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Co-aggregation of RNA binding proteins in ALS spinal motor neurons: evidence of a common pathogenic mechanism.

Keller BA, Volkening K, Droppelmann CA, Ang LC, Rademakers R, Strong MJ

While the pathogenesis of amyotrophic lateral sclerosis (ALS) remains to be clearly delineated, there is mounting evidence that altered RNA metabolism is a commonality amongst several of the known genetic variants of the disease. In this study, we evaluated the expression of 10 ALS-associated proteins in spinal motor neurons (MNs) in ALS patients with mutations in C9orf72 (C9orf72(GGGGCC)-ALS; n = 5), SOD1 ... [more]

Acta Neuropathol. Sep. 01, 2012; 0(0); [Pubmed: 22941224]

Throughput

  • Low Throughput

Additional Notes

  • figure 6.

Curated By

  • BioGRID