TOLLIP
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
PIAS2
Gene Ontology Biological Process
- androgen receptor signaling pathway [IMP]
- negative regulation of androgen receptor signaling pathway [ISO]
- positive regulation of dendrite morphogenesis [IMP]
- positive regulation of transcription from RNA polymerase II promoter [ISO]
- positive regulation of transcription, DNA-templated [IMP, ISO]
- protein sumoylation [ISO, ISS]
- regulation of androgen receptor signaling pathway [IDA]
- regulation of osteoblast differentiation [ISO]
- regulation of transcription from RNA polymerase II promoter [ISO]
- regulation of transcription, DNA-templated [IDA]
- response to organic substance [IEP]
- response to testosterone [IEP]
- spermatogenesis [IEP]
- transcription, DNA-templated [ISO]
Gene Ontology Molecular Function
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Tollip is a mediator of protein sumoylation.
Tollip is an interactor of the interleukin-1 receptor involved in its activation. The endosomal turnover of ubiquitylated IL-1RI is also controlled by Tollip. Furthermore, together with Tom1, Tollip has a general role in endosomal protein traffic. This work shows that Tollip is involved in the sumoylation process. Using the yeast two-hybrid technique, we have isolated new Tollip partners including two ... [more]
Throughput
- Low Throughput
Additional Notes
- figure 1.
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| TOLLIP PIAS2 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | 692441 | |
| TOLLIP PIAS2 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | 692428 |
Curated By
- BioGRID