RND2
Gene Ontology Biological Process
Gene Ontology Molecular Function
PLXNB1
Gene Ontology Biological Process
- axon guidance [TAS]
- cell migration [NAS]
- intracellular signal transduction [NAS]
- negative regulation of cell adhesion [IDA]
- negative regulation of osteoblast proliferation [ISS]
- neuron projection morphogenesis [ISS]
- ossification involved in bone maturation [ISS]
- positive regulation of Rho GTPase activity [ISS]
- positive regulation of phosphatidylinositol 3-kinase signaling [ISS]
- regulation of cell shape [IDA]
- regulation of cytoskeleton organization [IDA]
- semaphorin-plexin signaling pathway [IDA]
- semaphorin-plexin signaling pathway involved in bone trabecula morphogenesis [ISS]
- signal transduction [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
The GTPase-deficient Rnd proteins are stabilized by their effectors.
Rnd proteins are Rho family GTP binding proteins with cellular functions that antagonize RhoA signaling. We recently described a new Rnd3 effector Syx, also named PLEKHG5 that interacts with Rnds via a Raf1-like 'Ras binding domain'. Syx is a multi-domain RhoGEF that participates in early zebrafish development. Here we demonstrated that Rnd1, Rnd2 and Rnd3 stability is acutely dependent on ... [more]
Throughput
- Low Throughput
Additional Notes
- figure 5C.
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| PLXNB1 RND2 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - |
Curated By
- BioGRID