BAIT

RND2

ARHN, RHO7, RhoN
Rho family GTPase 2
GO Process (2)
GO Function (2)
GO Component (0)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

The GTPase-deficient Rnd proteins are stabilized by their effectors.

Goh LL, Manser E

Rnd proteins are Rho family GTP binding proteins with cellular functions that antagonize RhoA signaling. We recently described a new Rnd3 effector Syx, also named PLEKHG5 that interacts with Rnds via a Raf1-like 'Ras binding domain'. Syx is a multi-domain RhoGEF that participates in early zebrafish development. Here we demonstrated that Rnd1, Rnd2 and Rnd3 stability is acutely dependent on ... [more]

Unknown Jul. 17, 2012; 0(0); [Pubmed: 22807448]

Throughput

  • Low Throughput

Additional Notes

  • figure 5C.

Curated By

  • BioGRID