ATXN3
Gene Ontology Biological Process
- actin cytoskeleton organization [ISO]
- cellular response to heat [IMP]
- cellular response to misfolded protein [IMP]
- exploration behavior [IMP]
- intermediate filament cytoskeleton organization [ISO]
- microtubule cytoskeleton organization [ISO]
- misfolded or incompletely synthesized protein catabolic process [IMP]
- monoubiquitinated protein deubiquitination [IDA]
- proteasome-mediated ubiquitin-dependent protein catabolic process [IMP]
- protein K48-linked deubiquitination [ISO]
- protein K63-linked deubiquitination [ISO]
- proteolysis [ISO]
- regulation of cell-substrate adhesion [IMP, ISO]
- ubiquitin-dependent protein catabolic process [IMP]
Gene Ontology Molecular Function- ATPase binding [ISO]
- Lys48-specific deubiquitinase activity [ISO]
- Lys63-specific deubiquitinase activity [ISO]
- RNA polymerase II regulatory region DNA binding [ISO]
- histone deacetylase activity [ISO]
- identical protein binding [ISO]
- protein binding [IPI]
- ubiquitin protein ligase binding [IPI, ISO]
- ubiquitin thiolesterase activity [IDA]
- ubiquitin-specific protease activity [IDA]
- ATPase binding [ISO]
- Lys48-specific deubiquitinase activity [ISO]
- Lys63-specific deubiquitinase activity [ISO]
- RNA polymerase II regulatory region DNA binding [ISO]
- histone deacetylase activity [ISO]
- identical protein binding [ISO]
- protein binding [IPI]
- ubiquitin protein ligase binding [IPI, ISO]
- ubiquitin thiolesterase activity [IDA]
- ubiquitin-specific protease activity [IDA]
Gene Ontology Cellular Component
HSPA1B
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Misfolding promotes the ubiquitination of polyglutamine-expanded ataxin-3, the defective gene product in SCA3/MJD.
A major hallmark of the polyglutamine diseases is the formation of neuronal intranuclear inclusions (NIIs) of the disease proteins that are ubiquitinated and often associated with various chaperones and proteasome components. Recently, misfolding has come to be considered one of the primary factors for polyglutamine protein aggregation, although, the nature of misfolding and the relationship between misfolding and ubiquitination of ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID