CSF1R
Gene Ontology Biological Process
- cell proliferation [IMP]
- cell-cell junction maintenance [IMP]
- cellular response to cytokine stimulus [ISS]
- cellular response to macrophage colony-stimulating factor stimulus [IMP]
- cytokine-mediated signaling pathway [IMP]
- hemopoiesis [IMP]
- inflammatory response [TAS]
- macrophage differentiation [TAS]
- mammary gland duct morphogenesis [TAS]
- monocyte differentiation [TAS]
- multicellular organismal development [TAS]
- osteoclast differentiation [ISS]
- peptidyl-tyrosine phosphorylation [IDA]
- phosphatidylinositol metabolic process [ISS]
- phosphatidylinositol-mediated signaling [ISS]
- positive regulation of ERK1 and ERK2 cascade [ISS]
- positive regulation of cell migration [ISS]
- positive regulation of cell motility [IMP]
- positive regulation of cell proliferation [IMP]
- positive regulation of chemokine secretion [IMP]
- positive regulation of protein phosphorylation [IMP]
- positive regulation of protein serine/threonine kinase activity [ISS]
- positive regulation of protein tyrosine kinase activity [IMP]
- positive regulation of tyrosine phosphorylation of Stat3 protein [ISS]
- protein autophosphorylation [IDA]
- regulation of actin cytoskeleton reorganization [ISS]
- regulation of bone resorption [ISS]
- regulation of cell shape [IMP]
- ruffle organization [ISS]
- signal transduction [TAS]
- transmembrane receptor protein tyrosine kinase signaling pathway [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
STAP2
Gene Ontology Molecular Function
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
STAP-2 regulates c-Fms/M-CSF receptor signaling in murine macrophage Raw 264.7 cells.
Signal-transducing adaptor protein-2 (STAP-2) is a recently identified adaptor protein as a c-Fms/M-CSF receptor-interacting protein and constitutively expressed in macrophages. Our previous studies also revealed that STAP-2 binds to MyD88 and IKK-alpha/beta, and modulates NF-kappaB signaling in macrophages. In the present study, we examined physiological roles of the interaction between STAP-2 and c-Fms in Raw 264.7 macrophage cells. Our immunoprecipitation ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| STAP2 CSF1R | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - |
Curated By
- BioGRID