KSR1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
MAP2K1
Gene Ontology Biological Process
- Golgi inheritance [ISO]
- MAPK cascade [IMP, ISO]
- activation of MAPK activity [IMP, ISO, TAS]
- cell cycle arrest [ISO]
- cell motility [IMP]
- cell proliferation [ISO]
- cellular senescence [ISO]
- keratinocyte differentiation [IMP]
- labyrinthine layer development [IMP]
- melanosome transport [ISO]
- mitotic nuclear division [ISO]
- negative regulation of cell proliferation [ISO]
- negative regulation of homotypic cell-cell adhesion [ISO]
- neuron differentiation [IMP, ISO]
- neuron projection morphogenesis [ISO]
- peptidyl-tyrosine dephosphorylation [IMP]
- placenta blood vessel development [IMP]
- positive regulation of Ras GTPase activity [ISO]
- positive regulation of Ras protein signal transduction [ISO]
- positive regulation of cell differentiation [IDA]
- positive regulation of cell migration [ISO]
- positive regulation of gene expression [ISO]
- positive regulation of protein serine/threonine kinase activity [ISO]
- positive regulation of transcription elongation from RNA polymerase II promoter [ISO]
- protein heterooligomerization [ISO]
- protein phosphorylation [IMP, ISO, TAS]
- regulation of Golgi inheritance [TAS]
- regulation of early endosome to late endosome transport [TAS]
- regulation of stress-activated MAPK cascade [TAS]
- regulation of vascular smooth muscle contraction [ISO]
- response to axon injury [ISO]
- response to glucocorticoid [ISO]
- response to oxidative stress [ISO]
- vesicle transport along microtubule [ISO]
Gene Ontology Molecular Function- ATP binding [ISO]
- MAP kinase kinase activity [IMP, ISO]
- Ras GTPase binding [ISO]
- mitogen-activated protein kinase kinase kinase binding [ISO]
- protein C-terminus binding [ISO]
- protein N-terminus binding [ISO]
- protein binding [IPI]
- protein kinase binding [ISO]
- protein serine/threonine kinase activator activity [ISO]
- protein serine/threonine/tyrosine kinase activity [TAS]
- receptor signaling protein tyrosine phosphatase activity [IMP]
- ATP binding [ISO]
- MAP kinase kinase activity [IMP, ISO]
- Ras GTPase binding [ISO]
- mitogen-activated protein kinase kinase kinase binding [ISO]
- protein C-terminus binding [ISO]
- protein N-terminus binding [ISO]
- protein binding [IPI]
- protein kinase binding [ISO]
- protein serine/threonine kinase activator activity [ISO]
- protein serine/threonine/tyrosine kinase activity [TAS]
- receptor signaling protein tyrosine phosphatase activity [IMP]
Gene Ontology Cellular Component
- Golgi apparatus [ISO, TAS]
- axon [ISO]
- cell cortex [ISO]
- cytoplasm [ISO]
- cytosol [ISO, TAS]
- dendrite [ISO]
- dendrite cytoplasm [ISO]
- early endosome [TAS]
- endoplasmic reticulum [ISO]
- extracellular vesicular exosome [ISO]
- focal adhesion [TAS]
- late endosome [TAS]
- microtubule [ISO]
- mitochondrion [TAS]
- nucleus [TAS]
- perikaryon [ISO]
- perinuclear region of cytoplasm [ISO]
- plasma membrane [ISO]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
IMP modulates KSR1-dependent multivalent complex formation to specify ERK1/2 pathway activation and response thresholds.
The Ras effector and ubiquitin-protein isopeptide ligase family member IMP acts as a steady-state resistor within the Raf-MEK-ERK kinase module. IMP concentrations are regulated by Ras through induction of autodegradation and can modulate signal/response thresholds by directly limiting the assembly of functional KSR1-dependent Raf.MEK complexes. Here, we show that the capacity of IMP to inhibit signal propagation through Raf to ... [more]
Throughput
- Low Throughput
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
KSR1 MAP2K1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
KSR1 MAP2K1 | Biochemical Activity Biochemical Activity An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation. | Low | - | BioGRID | 715929 |
Curated By
- BioGRID