Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Obscurin and KCTD6 regulate cullin-dependent small ankyrin-1 (sAnk1.5) protein turnover.

Lange S, Perera S, Teh P, Chen J

Protein turnover through cullin-3 is tightly regulated by posttranslational modifications, the COP9-signalosome and BTB/POZ-domain proteins that link cullin-3 to specific substrates for ubiquitylation. Here we report how KCTD6 represents a novel substrate adaptor for cullin-3, effectively regulating protein levels of the muscle ankyrin-1 isoform sAnk1.5.Binding of sAnk1.5 to KCTD6, and its subsequent turnover is regulated through posttranslational modification by nedd8, ... [more]

Unknown May. 09, 2012; 0(0); [Pubmed: 22573887]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
ANK1 KCTD6
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID