ELSPBP1
UBE2I
Gene Ontology Biological Process
- negative regulation of transcription from RNA polymerase II promoter [ISO]
- negative regulation of transcription, DNA-templated [ISO]
- positive regulation of I-kappaB kinase/NF-kappaB signaling [ISO]
- positive regulation of intracellular steroid hormone receptor signaling pathway [IDA]
- positive regulation of sequence-specific DNA binding transcription factor activity [IDA]
- proteasome-mediated ubiquitin-dependent protein catabolic process [IMP]
- protein sumoylation [IDA, ISO]
- protein ubiquitination [IDA]
- regulation of receptor activity [IDA]
- ubiquitin-dependent protein catabolic process [TAS]
Gene Ontology Molecular Function- HLH domain binding [ISO]
- RING-like zinc finger domain binding [ISO]
- SUMO transferase activity [IDA, ISO]
- bHLH transcription factor binding [IPI]
- enzyme binding [ISO]
- poly(A) RNA binding [ISO]
- protein C-terminus binding [IPI]
- protein binding [IPI]
- transcription factor binding [ISO]
- ubiquitin protein ligase activity [IBA]
- ubiquitin protein ligase binding [IBA]
- ubiquitin-protein transferase activity [IDA]
- HLH domain binding [ISO]
- RING-like zinc finger domain binding [ISO]
- SUMO transferase activity [IDA, ISO]
- bHLH transcription factor binding [IPI]
- enzyme binding [ISO]
- poly(A) RNA binding [ISO]
- protein C-terminus binding [IPI]
- protein binding [IPI]
- transcription factor binding [ISO]
- ubiquitin protein ligase activity [IBA]
- ubiquitin protein ligase binding [IBA]
- ubiquitin-protein transferase activity [IDA]
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
Degradation of E2A proteins through a ubiquitin-conjugating enzyme, UbcE2A.
The helix-loop-helix E2A proteins (E12 and E47) govern cellular growth and differentiation. To identify binding partners that regulate the function of these ubiquitous transcription factors, we screened for proteins that interacted with the C terminus of E12 by the yeast interaction trap. UbcE2A, a rat enzyme that is highly homologous to and functionally complements the yeast ubiquitin-conjugating enzyme UBC9, was ... [more]
Throughput
- High Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| ELSPBP1 UBE2I | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low/High | - | BioGRID | - |
Curated By
- BioGRID