BAIT

CBLB

Cbl-b, RNF56, Nbla00127
Cbl proto-oncogene B, E3 ubiquitin protein ligase
GO Process (2)
GO Function (1)
GO Component (4)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens
PREY

PIK3R2

MPPH, MPPH1, P85B, p85, p85-BETA
phosphoinositide-3-kinase, regulatory subunit 2 (beta)
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

p85beta phosphoinositide 3-kinase regulates CD28 coreceptor function.

Alcazar I, Cortes I, Zaballos A, Hernandez C, Fruman DA, Barber DF, Carrera AC

CD28 is a receptor expressed on T cells that regulates their differentiation after antigen stimulation to long-term-survival memory T cells. CD28 enhances T-cell receptor signals and reduces expression of CBL ubiquitin ligases, which negatively control T-cell activation. In the absence of CD28 ligation during the primary stimulation, CBL levels remain high and T cells fail to mount an efficient secondary ... [more]

Blood Apr. 02, 2009; 113(14);3198-208 [Pubmed: 19190244]

Throughput

  • Low Throughput

Additional Notes

  • figure 3.

Curated By

  • BioGRID