An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Cbl-b-dependent degradation of FLIP(L) is involved in ATO-induced autophagy in leukemic K562 and gastric cancer cells.

Zhang G, Liu J, Zhang Y, Qu J, Xu L, Zheng H, Liu Y, Qu X

Various molecular mechanisms are involved in the efficacy of arsenic trioxide (ATO) against malignant hematologic and some solid tumors. FLICE-like inhibitory protein (FLIP) is an inhibitor of apoptosis mediated by death receptors. In this study, we identified a new link between the down-regulation of cellular FLIP(L) and ATO-induced autophagy. ATO induced the degradation of FLIP(L) in K562 and MGC803 cells, ... [more]

FEBS Lett. Aug. 01, 2012; 0(0); [Pubmed: 22884570]

Throughput

Low Throughput

Additional Notes

figure 4.

Curated By

BioGRID

Interaction Summary

CFLAR

Gene Ontology Biological Process

Gene Ontology Molecular Function

cysteine-type endopeptidase activity [IKR]death effector domain binding [IBA]enzyme activator activity [IDA]protease binding [IPI]protein binding [IPI]

Gene Ontology Cellular Component

CBLB