Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

CLIPR-59 regulates TNF-α-induced apoptosis by controlling ubiquitination of RIP1.

Fujikura D, Ito M, Chiba S, Harada T, Perez F, Reed JC, Uede T, Miyazaki T

Tumor necrosis factor-α (TNF-α) has important roles in several immunological events by regulating apoptosis and transcriptional activation of cytokine genes. Intracellular signaling mediated by TNF-receptor-type 1 (TNFR1) is constituted by two sequential protein complexes: Complex-I containing the receptor and Complex-II-containing Caspase-8. Protein modifications, particularly ubiquitination, are associated with the regulation of the formation of these complexes. However, the underlying mechanisms ... [more]

Cell Death Dis Feb. 03, 2012; 3(0);e264 [Pubmed: 22297296]

Throughput

  • Low Throughput

Curated By

  • BioGRID