BAIT

PRKCSH

80K-H, PKCSH
protein kinase C substrate 80K-H
Mus musculus
PREY

LPPR4

A330086D10, D3Bwg0562e, PRG-1, mKIAA0455
lipid phosphate phosphatase-related protein type 4
GO Process (3)
GO Function (2)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

TRIM67 protein negatively regulates Ras activity through degradation of 80K-H and induces neuritogenesis.

Yaguchi H, Okumura F, Takahashi H, Kano T, Kameda H, Uchigashima M, Tanaka S, Watanabe M, Sasaki H, Hatakeyama S

Tripartite motif (TRIM)-containing proteins, which are defined by the presence of a common domain structure composed of a RING finger, one or two B-box motifs and a coiled-coil motif, are involved in many biological processes including innate immunity, viral infection, carcinogenesis, and development. Here we show that TRIM67, which has a TRIM motif, an FN3 domain and a SPRY domain, ... [more]

J. Biol. Chem. Apr. 06, 2012; 287(15);12050-9 [Pubmed: 22337885]

Throughput

  • Low Throughput

Additional Notes

  • figure 2.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
LPPR4 PRKCSH
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
693432

Curated By

  • BioGRID