BAIT

CSK

AW212630
c-src tyrosine kinase
GO Process (25)
GO Function (9)
GO Component (7)
Mus musculus
PREY

CREBBP

AW558298, CBP, CBP/p300, KAT3A, p300/CBP
CREB binding protein
GO Process (11)
GO Function (19)
GO Component (10)
Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Targeting Lyn tyrosine kinase through protein fusions encompassing motifs of Cbp (Csk-binding protein) and the SOCS box of SOCS1.

Whiting RJ, Payne CJ, Satiaputra J, Kucera N, Qiu TW, Irtegun S, Gunn NJ, Slavova-Azmanova NS, Lavova-Azmanova NS, Mulhern TD, Ingley E

The tyrosine kinase Lyn is involved in oncogenic signalling in several leukaemias and solid tumours, and we have previously identified a pathway centred on Cbp [Csk (C-terminal Src kinase)-binding protein] that mediates both enzymatic inactivation, as well as proteasomal degradation of Lyn via phosphorylation-dependent recruitment of Csk (responsible for phosphorylating the inhibitory C-terminal tyrosine of Lyn) and SOCS1 (suppressor of ... [more]

Biochem. J. Mar. 15, 2012; 442(3);611-20 [Pubmed: 22364282]

Throughput

  • Low Throughput

Additional Notes

  • figure 3.

Curated By

  • BioGRID