BAIT
LNX1
LNX, MPDZ, PDZRN2, UNQ574/PRO1136
ligand of numb-protein X 1, E3 ubiquitin protein ligase
GO Process (0)
GO Function (1)
GO Component (0)
Gene Ontology Molecular Function
Homo sapiens
PREY
ARHGAP6
RHOGAP6, RHOGAPX-1
Rho GTPase activating protein 6
GO Process (10)
GO Function (4)
GO Component (4)
Gene Ontology Biological Process
- Rho protein signal transduction [NAS]
- actin filament polymerization [NAS]
- activation of phospholipase C activity [IDA]
- negative regulation of focal adhesion assembly [IMP]
- negative regulation of stress fiber assembly [IMP]
- positive regulation of phospholipase activity [IDA]
- positive regulation of signal transduction [TAS]
- regulation of GTPase activity [ISS]
- regulation of small GTPase mediated signal transduction [TAS]
- small GTPase mediated signal transduction [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
Proteomics Strategy to Identify Substrates of LNX, a PDZ Domain-containing E3 Ubiquitin Ligase.
Ubiquitin ligases (E3s) confer specificity to ubiquitination by recognizing target substrates. However, the substrates of most E3s have not been extensively discovered, and new methods are needed to efficiently and comprehensively identify these substrates. Mostly, E3s specifically recognize substrates via their protein interaction domains. We developed a novel integrated strategy to identify substrates of E3s containing protein interaction domains on ... [more]
J. Proteome Res. Aug. 29, 2012; 0(0); [Pubmed: 22889411]
Throughput
- Low Throughput
Additional Notes
- table 2.
Curated By
- BioGRID