BAIT
LNX1
LNX, MPDZ, PDZRN2, UNQ574/PRO1136
ligand of numb-protein X 1, E3 ubiquitin protein ligase
GO Process (0)
GO Function (1)
GO Component (0)
Gene Ontology Molecular Function
Homo sapiens
PREY
ARHGEF16
GEF16, NBR, RP11-168F9.1
Rho guanine nucleotide exchange factor (GEF) 16
GO Process (9)
GO Function (5)
GO Component (1)
Gene Ontology Biological Process
- activation of Cdc42 GTPase activity [IDA]
- activation of Rac GTPase activity [IMP]
- apoptotic signaling pathway [TAS]
- cell chemotaxis [IMP]
- neurotrophin TRK receptor signaling pathway [TAS]
- positive regulation of apoptotic process [TAS]
- positive regulation of establishment of protein localization to plasma membrane [IMP]
- regulation of small GTPase mediated signal transduction [TAS]
- small GTPase mediated signal transduction [TAS]
Gene Ontology Molecular Function
Homo sapiens
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
Proteomics Strategy to Identify Substrates of LNX, a PDZ Domain-containing E3 Ubiquitin Ligase.
Ubiquitin ligases (E3s) confer specificity to ubiquitination by recognizing target substrates. However, the substrates of most E3s have not been extensively discovered, and new methods are needed to efficiently and comprehensively identify these substrates. Mostly, E3s specifically recognize substrates via their protein interaction domains. We developed a novel integrated strategy to identify substrates of E3s containing protein interaction domains on ... [more]
J. Proteome Res. Aug. 29, 2012; 0(0); [Pubmed: 22889411]
Throughput
- Low Throughput
Additional Notes
- table 2.
Curated By
- BioGRID