ADRA2A
Gene Ontology Biological Process
- G-protein coupled receptor signaling pathway [ISS]
- Ras protein signal transduction [TAS]
- Rho protein signal transduction [TAS]
- actin cytoskeleton organization [TAS]
- activation of MAPK activity by adrenergic receptor signaling pathway [IDA]
- activation of protein kinase B activity [IDA]
- activation of protein kinase activity [IDA]
- adenylate cyclase-inhibiting adrenergic receptor signaling pathway [IDA]
- blood coagulation [TAS]
- cellular component movement [TAS]
- cellular response to hormone stimulus [IGI]
- energy reserve metabolic process [TAS]
- epidermal growth factor-activated receptor transactivation by G-protein coupled receptor signaling pathway [IDA]
- glucose homeostasis [IMP]
- intestinal absorption [TAS]
- negative regulation of adenylate cyclase activity [ISS]
- negative regulation of adrenergic receptor signaling pathway [ISS]
- negative regulation of cAMP biosynthetic process [IDA, ISS]
- negative regulation of calcium ion transmembrane transporter activity [ISS]
- negative regulation of calcium ion transport [ISS]
- negative regulation of calcium ion-dependent exocytosis [IC]
- negative regulation of epinephrine secretion [NAS]
- negative regulation of insulin secretion [IMP]
- negative regulation of insulin secretion involved in cellular response to glucose stimulus [IMP]
- negative regulation of lipid catabolic process [IGI]
- negative regulation of norepinephrine secretion [NAS, TAS]
- phospholipase C-activating adrenergic receptor signaling pathway [IDA]
- platelet activation [TAS]
- positive regulation of MAP kinase activity [IDA]
- positive regulation of cell migration [IMP]
- positive regulation of cell proliferation [TAS]
- positive regulation of cytokine production [IDA]
- positive regulation of epidermal growth factor-activated receptor activity [IDA]
- positive regulation of membrane protein ectodomain proteolysis [IDA]
- positive regulation of potassium ion transport [ISS]
- positive regulation of wound healing [IMP]
- regulation of insulin secretion [TAS]
- signal transduction [TAS]
- small molecule metabolic process [TAS]
Gene Ontology Molecular Function- alpha-1B adrenergic receptor binding [ISS]
- alpha-2C adrenergic receptor binding [IPI]
- alpha2-adrenergic receptor activity [IDA, ISS]
- epinephrine binding [IDA]
- heterotrimeric G-protein binding [IDA]
- norepinephrine binding [IDA]
- protein binding [IPI]
- protein heterodimerization activity [IPI, ISS]
- protein homodimerization activity [IDA]
- protein kinase binding [IPI]
- thioesterase binding [IPI]
- alpha-1B adrenergic receptor binding [ISS]
- alpha-2C adrenergic receptor binding [IPI]
- alpha2-adrenergic receptor activity [IDA, ISS]
- epinephrine binding [IDA]
- heterotrimeric G-protein binding [IDA]
- norepinephrine binding [IDA]
- protein binding [IPI]
- protein heterodimerization activity [IPI, ISS]
- protein homodimerization activity [IDA]
- protein kinase binding [IPI]
- thioesterase binding [IPI]
Gene Ontology Cellular Component
UCHL1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
Interaction of the ubiquitin carboxyl terminal esterase L1 with alpha(2)-adrenergic receptors inhibits agonist-mediated p44/42 MAP kinase activation.
Neuroprotective effects of alpha(2)-adrenergic receptor (AR) agonists are mediated via the alpha(2A)AR subtype, but the molecular mechanisms underlying these actions are still not elucidated. A two-hybrid screen was performed to identify new proteins that may control alpha(2)AR receptor function and trafficking. This screen identified the ubiquitin carboxyl-terminal hydrolase-L1 (Uch-L1), a protein associated with Parkinson's disease, as alpha(2)AR interacting protein. This ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| ADRA2A UCHL1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| ADRA2A UCHL1 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID