VCP
Gene Ontology Biological Process
- ATP catabolic process [IDA, IMP]
- ER to Golgi vesicle-mediated transport [IMP]
- ER-associated ubiquitin-dependent protein catabolic process [ISO, ISS]
- activation of cysteine-type endopeptidase activity involved in apoptotic process [ISO]
- aggresome assembly [ISO]
- cellular response to DNA damage stimulus [ISO, ISS]
- double-strand break repair [ISO, ISS]
- positive regulation of Lys63-specific deubiquitinase activity [ISO]
- positive regulation of proteasomal ubiquitin-dependent protein catabolic process [IMP, ISO]
- positive regulation of protein K63-linked deubiquitination [ISO]
- positive regulation of protein catabolic process [ISO]
- positive regulation of protein complex assembly [ISO]
- protein N-linked glycosylation via asparagine [ISO, ISS]
- protein hexamerization [IDA]
- protein homooligomerization [IDA]
- protein ubiquitination [ISO, ISS]
- retrograde protein transport, ER to cytosol [ISO]
- translesion synthesis [ISO, ISS]
- ubiquitin-dependent protein catabolic process [ISO]
Gene Ontology Molecular Function- ADP binding [IMP]
- ATP binding [IDA, IMP]
- ATPase activity [IDA, IMP]
- deubiquitinase activator activity [ISO]
- identical protein binding [IDA]
- poly(A) RNA binding [ISO]
- polyubiquitin binding [ISO]
- protein binding [IPI]
- protein complex binding [IPI]
- protein domain specific binding [ISO]
- protein phosphatase binding [ISO]
- receptor binding [IDA]
- ubiquitin-specific protease binding [ISO]
- ADP binding [IMP]
- ATP binding [IDA, IMP]
- ATPase activity [IDA, IMP]
- deubiquitinase activator activity [ISO]
- identical protein binding [IDA]
- poly(A) RNA binding [ISO]
- polyubiquitin binding [ISO]
- protein binding [IPI]
- protein complex binding [IPI]
- protein domain specific binding [ISO]
- protein phosphatase binding [ISO]
- receptor binding [IDA]
- ubiquitin-specific protease binding [ISO]
Gene Ontology Cellular Component
- Hrd1p ubiquitin ligase complex [ISO]
- cytoplasm [ISO]
- cytosol [IDA, ISO]
- endoplasmic reticulum [IDA, ISO]
- endoplasmic reticulum membrane [ISO]
- extracellular vesicular exosome [ISO]
- intracellular membrane-bounded organelle [IDA]
- lipid particle [ISO]
- myelin sheath [ISO]
- nucleoplasm [ISO]
- nucleus [ISO]
- perinuclear region of cytoplasm [ISO]
- proteasome complex [ISO]
- protein complex [ISO]
- site of double-strand break [ISO, ISS]
VCP
Gene Ontology Biological Process
- ATP catabolic process [IDA, IMP]
- ER to Golgi vesicle-mediated transport [IMP]
- ER-associated ubiquitin-dependent protein catabolic process [ISO, ISS]
- activation of cysteine-type endopeptidase activity involved in apoptotic process [ISO]
- aggresome assembly [ISO]
- cellular response to DNA damage stimulus [ISO, ISS]
- double-strand break repair [ISO, ISS]
- positive regulation of Lys63-specific deubiquitinase activity [ISO]
- positive regulation of proteasomal ubiquitin-dependent protein catabolic process [IMP, ISO]
- positive regulation of protein K63-linked deubiquitination [ISO]
- positive regulation of protein catabolic process [ISO]
- positive regulation of protein complex assembly [ISO]
- protein N-linked glycosylation via asparagine [ISO, ISS]
- protein hexamerization [IDA]
- protein homooligomerization [IDA]
- protein ubiquitination [ISO, ISS]
- retrograde protein transport, ER to cytosol [ISO]
- translesion synthesis [ISO, ISS]
- ubiquitin-dependent protein catabolic process [ISO]
Gene Ontology Molecular Function- ADP binding [IMP]
- ATP binding [IDA, IMP]
- ATPase activity [IDA, IMP]
- deubiquitinase activator activity [ISO]
- identical protein binding [IDA]
- poly(A) RNA binding [ISO]
- polyubiquitin binding [ISO]
- protein binding [IPI]
- protein complex binding [IPI]
- protein domain specific binding [ISO]
- protein phosphatase binding [ISO]
- receptor binding [IDA]
- ubiquitin-specific protease binding [ISO]
- ADP binding [IMP]
- ATP binding [IDA, IMP]
- ATPase activity [IDA, IMP]
- deubiquitinase activator activity [ISO]
- identical protein binding [IDA]
- poly(A) RNA binding [ISO]
- polyubiquitin binding [ISO]
- protein binding [IPI]
- protein complex binding [IPI]
- protein domain specific binding [ISO]
- protein phosphatase binding [ISO]
- receptor binding [IDA]
- ubiquitin-specific protease binding [ISO]
Gene Ontology Cellular Component
- Hrd1p ubiquitin ligase complex [ISO]
- cytoplasm [ISO]
- cytosol [IDA, ISO]
- endoplasmic reticulum [IDA, ISO]
- endoplasmic reticulum membrane [ISO]
- extracellular vesicular exosome [ISO]
- intracellular membrane-bounded organelle [IDA]
- lipid particle [ISO]
- myelin sheath [ISO]
- nucleoplasm [ISO]
- nucleus [ISO]
- perinuclear region of cytoplasm [ISO]
- proteasome complex [ISO]
- protein complex [ISO]
- site of double-strand break [ISO, ISS]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Functional ATPase activity of p97/valosin-containing protein (VCP) is required for the quality control of endoplasmic reticulum in neuronally differentiated mammalian PC12 cells.
Abnormal protein accumulation and cell death with cytoplasmic vacuoles are hallmarks of several neurodegenerative disorders. We previously identified p97/valosin-containing protein (VCP), an AAA ATPase with two conserved ATPase domains (D1 and D2), as an interacting partner of the Machado-Joseph disease (MJD) protein with expanded polyglutamines that causes Machado-Joseph disease. To reveal its pathophysiological roles in neuronal cells, we focused on ... [more]
Throughput
- Low Throughput
Additional Notes
- p97 also interacted with p97(K524A)mutant
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| VCP VCP | Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex. | Low | - | BioGRID | 695437 | |
| VCP VCP | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - |
Curated By
- BioGRID