UBC
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
VCP
Gene Ontology Biological Process
- ATP catabolic process [ISO]
- ER to Golgi vesicle-mediated transport [ISO]
- ER-associated ubiquitin-dependent protein catabolic process [ISO]
- activation of cysteine-type endopeptidase activity involved in apoptotic process [IDA]
- aggresome assembly [IGI]
- cellular response to DNA damage stimulus [ISO]
- double-strand break repair [ISO]
- positive regulation of Lys63-specific deubiquitinase activity [ISO]
- positive regulation of proteasomal ubiquitin-dependent protein catabolic process [ISO]
- positive regulation of protein K63-linked deubiquitination [ISO]
- positive regulation of protein catabolic process [ISO]
- positive regulation of protein complex assembly [ISO]
- protein N-linked glycosylation via asparagine [ISO]
- protein hexamerization [ISO]
- protein homooligomerization [ISO]
- protein ubiquitination [ISO]
- retrograde protein transport, ER to cytosol [ISO]
- translesion synthesis [ISO]
- ubiquitin-dependent protein catabolic process [IGI]
Gene Ontology Molecular Function- ADP binding [ISO]
- ATP binding [ISO]
- ATPase activity [ISO]
- deubiquitinase activator activity [ISO]
- identical protein binding [ISO]
- poly(A) RNA binding [ISO]
- polyubiquitin binding [IDA, ISO]
- protein binding [IPI]
- protein complex binding [ISO]
- protein domain specific binding [ISO]
- protein phosphatase binding [ISO]
- receptor binding [ISO]
- ubiquitin-specific protease binding [ISO]
- ADP binding [ISO]
- ATP binding [ISO]
- ATPase activity [ISO]
- deubiquitinase activator activity [ISO]
- identical protein binding [ISO]
- poly(A) RNA binding [ISO]
- polyubiquitin binding [IDA, ISO]
- protein binding [IPI]
- protein complex binding [ISO]
- protein domain specific binding [ISO]
- protein phosphatase binding [ISO]
- receptor binding [ISO]
- ubiquitin-specific protease binding [ISO]
Gene Ontology Cellular Component
- Hrd1p ubiquitin ligase complex [ISO]
- cytoplasm [ISO]
- cytosol [ISO]
- endoplasmic reticulum [ISO]
- endoplasmic reticulum membrane [ISO]
- extracellular vesicular exosome [ISO]
- intracellular membrane-bounded organelle [ISO]
- lipid particle [ISO]
- myelin sheath [IDA]
- nucleoplasm [ISO]
- nucleus [ISO]
- perinuclear region of cytoplasm [ISO]
- proteasome complex [ISO]
- protein complex [IPI]
- site of double-strand break [ISO]
Reconstituted Complex
An interaction is detected between purified proteins in vitro.
Publication
Direct binding of ubiquitin conjugates by the mammalian p97 adaptor complexes, p47 and Ufd1-Npl4.
The multiple functions of the p97/Cdc48p ATPase can be explained largely by adaptors that link its activity to different cellular pathways, but how these adaptors recognize different substrates is unclear. Here we present evidence that the mammalian adaptors, p47 and Ufd1-Npl4, both bind ubiquitin conjugates directly and so link p97 to ubiquitylated substrates. In the case of Ufd1-Npl4, which is ... [more]
Throughput
- Low Throughput
Additional Notes
- #LPPI
- Likely protein-protein interaction
- figure 2. Interaction much stronger in the presence of adaptors, Ufd1 and Nploc4 or p47 (Nsfl1c)
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| UBC VCP | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | 879324 | |
| UBC VCP | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | 1108280 | |
| UBC VCP | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | 2513462 | |
| UBC VCP | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | High | - | BioGRID | 2297254 |
Curated By
- BioGRID