Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

PTPN14 is required for the density-dependent control of YAP1.

Wang W, Huang J, Wang X, Yuan J, Li X, Feng L, Park JI, Chen J

Through an shRNA-mediated loss-of-function screen, we identified PTPN14 as a potential tumor suppressor. PTPN14 interacts with yes-associated protein 1 (YAP1), a member of the hippo signaling pathway. We showed that PTPN14 promotes the nucleus-to-cytoplasm translocation of YAP1 during contact inhibition and thus inhibits YAP1 transactivation activity. Interestingly, PTPN14 protein stability was positively controlled by cell density. We identified the CRL2(LRR1) ... [more]

Genes Dev. Sep. 01, 2012; 26(17);1959-71 [Pubmed: 22948661]

Throughput

  • Low Throughput

Additional Notes

  • PTPN14 interacts with the CCRL2-LRR1 E3 ligase.

Curated By

  • BioGRID