BAIT

VAMP2

SYB2, VAMP-2
vesicle-associated membrane protein 2 (synaptobrevin 2)
GO Process (29)
GO Function (9)
GO Component (25)
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

E3 ubiquitin ligase RNF13 involves spatial learning and assembly of the SNARE complex.

Zhang Q, Li Y, Zhang L, Yang N, Meng J, Zuo P, Zhang Y, Chen J, Wang L, Gao X, Zhu D

Changes in the structure and number of synapses modulate learning, memory and cognitive disorders. Ubiquitin-mediated protein modification is a key mechanism for regulating synaptic activity, though the precise control of this process remains poorly understood. RING finger protein 13 (RNF13) is a recently identified E3 ubiquitin ligase, and its in vivo function remains completely unknown. We show here that genetic ... [more]

Cell. Mol. Life Sci. Aug. 14, 2012; 0(0); [Pubmed: 22890573]

Throughput

  • Low Throughput

Curated By

  • BioGRID