B2M
Gene Ontology Biological Process
- T cell differentiation in thymus [ISO]
- antigen processing and presentation of exogenous protein antigen via MHC class Ib, TAP-dependent [ISO]
- iron ion homeostasis [ISO]
- positive regulation of T cell mediated cytotoxicity [ISO]
- protein refolding [ISO]
- response to cadmium ion [IEP]
- response to drug [IEP]
- response to molecule of bacterial origin [ISO]
- retina homeostasis [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
UBC
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Function of the p97-Ufd1-Npl4 complex in retrotranslocation from the ER to the cytosol: dual recognition of nonubiquitinated polypeptide segments and polyubiquitin chains.
A member of the family of ATPases associated with diverse cellular activities, called p97 in mammals and Cdc48 in yeast, associates with the cofactor Ufd1-Npl4 to move polyubiquitinated polypeptides from the endoplasmic reticulum (ER) membrane into the cytosol for their subsequent degradation by the proteasome. Here, we have studied the mechanism by which the p97-Ufd1-Npl4 complex functions in this retrotranslocation ... [more]
Throughput
- Low Throughput
Additional Notes
- figure 8.
Curated By
- BioGRID