BAIT
IRF2
9830146E22Rik, AI646973, Irf-2
interferon regulatory factor 2
GO Process (3)
GO Function (4)
GO Component (3)
Gene Ontology Biological Process
Gene Ontology Molecular Function- DNA binding [ISO]
- RNA polymerase II regulatory region sequence-specific DNA binding [ISO]
- RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [ISO]
- sequence-specific DNA binding transcription factor activity [ISO]
- DNA binding [ISO]
- RNA polymerase II regulatory region sequence-specific DNA binding [ISO]
- RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [ISO]
- sequence-specific DNA binding transcription factor activity [ISO]
Gene Ontology Cellular Component
Mus musculus
PREY
UBE2I
5830467E05Rik, F830028O17Rik, UBC9, Ubce2i, Ubce9
ubiquitin-conjugating enzyme E2I
GO Process (10)
GO Function (10)
GO Component (8)
Gene Ontology Biological Process
- cellular protein modification process [TAS]
- negative regulation of transcription from RNA polymerase II promoter [IMP, ISO]
- negative regulation of transcription, DNA-templated [ISO]
- positive regulation of I-kappaB kinase/NF-kappaB signaling [IDA]
- positive regulation of intracellular steroid hormone receptor signaling pathway [ISO]
- positive regulation of sequence-specific DNA binding transcription factor activity [ISO]
- proteasome-mediated ubiquitin-dependent protein catabolic process [ISO]
- protein sumoylation [IBA, ISO]
- protein ubiquitination [IBA, ISO]
- regulation of receptor activity [ISO]
Gene Ontology Molecular Function- HLH domain binding [IPI]
- RING-like zinc finger domain binding [ISO]
- SUMO transferase activity [IBA, ISO]
- bHLH transcription factor binding [ISO]
- enzyme binding [ISO]
- poly(A) RNA binding [ISO]
- protein C-terminus binding [ISO]
- protein binding [IPI]
- transcription factor binding [ISO]
- ubiquitin-protein transferase activity [ISO]
- HLH domain binding [IPI]
- RING-like zinc finger domain binding [ISO]
- SUMO transferase activity [IBA, ISO]
- bHLH transcription factor binding [ISO]
- enzyme binding [ISO]
- poly(A) RNA binding [ISO]
- protein C-terminus binding [ISO]
- protein binding [IPI]
- transcription factor binding [ISO]
- ubiquitin-protein transferase activity [ISO]
Mus musculus
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
Ubc9-mediated sumoylation leads to transcriptional repression of IRF-1.
To characterize the regulatory mechanism of the interferon regulatory factor (IRF) family, we performed yeast two-hybrid screening with IRF-2 and isolated the small ubiquitin-related modifier (SUMO)-conjugating enzyme Ubc9, which also interacts with other IRF family members IRF-1 and ICSBP. Subsequent assays indicated that among the IRF family members, only IRF-1 interacts with SUMO-1 through its transcriptional activation domain. The interaction ... [more]
Biochem. Biophys. Res. Commun. Dec. 19, 2008; 377(3);952-6 [Pubmed: 18955028]
Throughput
- Low Throughput
Curated By
- BioGRID